WebEach lot of this antibody is quality control tested by neutralization of proliferation induced by recombinant mouse IL-1β (Cat. No. 575102) at 0.05 ng/mL on D10.G4.1 mouse helper … Webd10 Cells. d10 Cells. Organism Type: Mouse: Full name is D10G4.1. This is a Th2 helper cell line, derived from AKR/J (H-2K) mice, specific for egg white conalbumin. Tissues: Lymphocyte: ... Every step of the way, a wide range of cell health products. Maintaining healthy cells is the key to experimental success and reproducible research results ...
IL-1 RI Antibody (JAMA-147) - BSA Free - Novus Biologicals
WebJun 22, 2024 · Briefly, D10.G4.1 cells (2 x 10 6 cells/well) were dispensed into anti-CD3 mAb coated 6-well plates, with or without varying concentrations of NK-4, and incubated for 6 h at 37°C. Total RNA was extracted from D10.G4.1 cells using an RNeasy Mini kit (QIAGEN, Tokyo, Japan) and DNase (QIAGEN) according to the manufacturer’s … WebMeasured by the ability of the immobilized protein to support the adhesion of D10.G4.1 mouse helper T cells. The ED 50 for this effect is 0.6-3 μg/mL. Source E. coli -derived mouse Galectin-9 protein Ala2-Thr322 … improve your fps
D10 G4 1 Cell Line ATCC Bioz
WebED 50 10.0 pg/ml, measured by the dose-dependent stimulation of mouse D10.G4.1 helper T cells, ... keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte ... WebRecombinant Mouse IL-1 alpha /IL-1F1 (Catalog # 400-ML) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED 50 for this effect is 3-7 pg/mL. SDS … WebMeasured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED 50 for this effect is typically 2-10 pg/mL. 2. Measured by its binding ability in a functional ELISA. 3. Immobilized mouse IL1A at 10 μg/mL (100 μl/well) can bind mouse IL1R1, The EC 50 of mouse IL1R1 is 0.13 μg/mL. improve your fishing magazine