Phospho ck2 antibody
WebOct 8, 2007 · Here we describe a new phospho-specific antibody against Cdc37 that recognizes recombinant purified Cdc37 only when incubated with CK2 in the presence of Mg 2+ and ATP. The replacement of Ser13 in Cdc37 by nonphosphorylatable amino acids abolished binding to this antibody. WebJun 9, 2024 · CK2 is an evolutionarily conserved serine/threonine kinase and is a heterotetramer composed of two catalytic α subunits and two regulatory β subunits. The α subunit can phosphorylate substrates as part of the tetrameric complex or as a monomer ( Grankowski et al., 1991 ).
Phospho ck2 antibody
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WebApr 11, 2024 · Bisphenol-A (BPA) is a common environmental toxicant that is known to be associated with fetal growth restriction (FGR). However, the mechanisms of how BPA induce FGR is poorly characterized. We conducted proteomics to identify the abnormal expression of SRB1 in female placental tissues with high BPA-induced FGR and further verified its … WebDec 26, 2024 · The CCA cell lines all showed a reduction in multiple phospho-CK2 substrates with apparent molecular masses of approximately 200, 180, 100, 60, 37, 35, and 33 kDa as indicated by arrows in the Figure ... We measured CK2 activity indirectly by using an antibody against the phospho-CK2 substrate (motif pS/pTDXE) to measure the amount of ...
WebMar 14, 2024 · It is noted that CK2 was also identified as the major kinase in the dominant core phosphoprotein network with higher basal level of phosphorylation stoichiometry in resistant NSCLC cells 21. Therefore, CK2 may be an attractive candidate for developing a novel therapeutic strategy for EGFR-TKI resistance lung cancer. WebThe antibody was specific for phosphorylated Cdc37 and did not crossreact with other CK2 substrates such as Hsp90 and FK506-binding protein 52. Using this antibody, we showed that complexes of Hsp90 with its client signaling kinases, Cdk4, MOK, v-Src, and Raf1, contained the CK2-phosphorylated form of Cdc37 in vivo.
WebPhospho-CK2 alpha-1 (Tyr255) Antibody, PA5-38831, from Invitrogen™. Species Reactivity: Human, Mouse, Rat; Applications: Western Blot Shop Phospho-CK2 alpha-1 (Tyr255) … WebCasein kinase II α(CK2) is a pleiotropic serine/threonine kinase, that is located on human chromosome 16p13. 2-p13. 3. Immunogen The antiserum was produced against …
WebThe antibody was specific for phosphorylated Cdc37 and did not crossreact with other CK2 substrates such as Hsp90 and FK506-binding protein 52. Using this antibody, we showed …
WebMar 15, 2024 · A likely explanation is that the increment of these phosphosites reflects indirect consequences of CK2 inhibition affecting the activity of other protein kinases and/or phosphatases in some way under the control od CK2 signaling. Download : Download high-res image (1MB) Download : Download full-size image Fig. 3. Sequence logo analyses. A. ina birthday merchWebPhosphospecific antibodies are affinity-purified rabbit polyclonal or monoclonal antibodies that are monospecific for a target protein that is phosphorylated. These antibodies provide critical insight into the investigation of complex signal transductions events. On this page Why use phosphospecific antibodies? incense crafting \u0026 use of magickal scentsWebDec 22, 2024 · CK2 kinase activity is often determined by measuring the phosphorylation of one of the down-stream signaling molecules, namely Akt, which is phosphorylated by CK2 at serine129 . Phosphorylation of Akt is detected by a phospho-specific antibody directed against the CK2 phosphorylation site serine 129. ina best recipesWebA phospho specific peptide corresponding to residues surrounding S1981 of human ATM Storage Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. ina birthday streamWebCasein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, … incense cypress treesWebemploys a phospho-residue (Tyr, Ser, Thr) motif antibody for phospho-peptide immunoaffinity purification from cell extracts combined with LC tandem MS/MS to identify and quantify changes in phosphorylation levels (1). Casein Kinase II (CK2) is a highly conserved, ubiquitously expressed, and incense day pokemon go 2021Web(A) SK-N-SH cells transfected with myc-HSJ1a WT or Ser250Ala mutant were stained with anti-c-myc or anti-phospho HSJ1 antibody, as indicated, and detected by the Alexa Fluor 488 secondary antibody ... ina birthday wallpaper